Dye-sensitized solar cell (DSSC) is a new type of solar cell that has attracted interest due to its ability to convert energy at a low cost, with simple fabrication, and non-toxic nature. Utilisation of oyster mushroom dye as a natural dye for DSSC is attributed to their large biomass yield and the fact that they can be cultivated in a regulated environment. The TiO2 pH6 was then used to study different deposition layer thicknesses of TiO2 thin film (1 layer, 2 layers, 3 layers, 4 layers and 5 layers) to suggest the optimised TiO2 condition. The characterization of the structural, morphological, optical, and electrical properties of the TiO2 photoanode with mushroom dye was carried out using XRD, FESEM, AFM, UV–Vis, and I-V measurements in this study, respectively. It was found that the pink oyster mushroom, dried by freeze drying with a 1:10 dilution and pH6 TiO2 with 3 deposition layers, established the best results in the solar cell performance with efficiency of 0.107 % along with the optimum bandgap of 1.50 eV and anatase structural phase of (101)-planes.