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Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors
  We have constructed recombinant baculoviruses containing cDNAs encoding either the [alpha]1- or the [beta]1-subunit of the bovine GABAA receptor. In Spodoptera frugiperda (IPLB-Sf-21) cells infected with recombinant virus expressing either the [alpha]1- or [beta]1-subunit, or in cells co-infected with both viruses, functional GABAA receptors were detected by whole-cell electrophysiological recordings. The threshold for the responses mediated by the homo-oligomeric channels ([alpha]- or [beta]-) was 2-3 X 10-6 M GABA, and for the co-infected cells was 8 X 10-8 M GABA, suggesting that hetero-oligomeric channels formed in these cells. All GABA-induced currents were found to be inhibited by bicuculline and picrotoxin, potentiated by pentobarbital but were insensitive to benzodiazepines.

  • Type:

    Article

  • Date:

    31 July 1992

  • Publication Status:

    Published

  • Publisher

    Ovid Technologies (Wolters Kluwer Health)

  • DOI:

  • ISSN:

    0959-4965

  • Library of Congress:

    QH301 Biology

  • Dewey Decimal Classification:

    572 Biochemistry

Citation

麻豆社区

Atkinson, A. E., Bermudez, I., Darlison, M. G., Barnard, E. A., Earley, F. G. P., Possee, R. D., 鈥ing, L. A. (1992). Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors. NeuroReport, 3(7), 597-600. https://doi.org/10.1097/00001756-199207000-00013

Keywords

GABAA receptor, Baculovirus expression;, Insect cells

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